pAVE1


Vector IG Sequence Link :
General : plasmid ds-DNA 2787 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)(monkey COS-7)
Suppliers : (ATCC)
Misc.Comments : Shuttle expression vector. Constructed by ligating the larger HindIII/EcoRI fragment of pCMVcat (removing CAT and the SV40 polyadenylation signals) to the HindIII/EcoRI multiple cloning site of pUC19. Commercial use of the human cytomaglovirus promoter/enhancer has been patented on behalf of B. Fleckenstein and W. Schaffner by the Beringwerke. Interested parties should contact the patent holders before planning a commercial application. Restriction digests of the clone give the following sizes (kb): BamHI--3.1; PstI--2.3, 0.8; XbaI--2.4, 0.7. (ATCC staff) The order of the major features in this plasmid is: hCMV enhancer/promoter - HindIII - MCS - EcoRI - bla - ori. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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