Vector IG Sequence Link :
General : plasmid ds-DNA 4070 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli RR1)(vertebrate cells)(E.coli)(monkey COS-1 cell line)(mammal cell line)(avian cell line)
Suppliers : (ATCC)
Misc.Comments : cDNA clones without an initiation codon may be cloned in frame into the unique BamHI site 3' to the AUG of the rat preproinsulin gene. This results in 5 - 6 additional amino terminal amino acids from the insulin signal peptide. [1] Genomic sequences are normally inserted between unique HindIII and SmaI sites (5' to 3'). This deletes the preproinsulin gene from the vector. [1] cDNA sequences with a translation initiation codon are usually inserted between the unique HindIII and BamHI sites (5' to 3'). [1] Polyadenylation signals (and possibly carboxy terminal amino acids) are contributed by the preproinsulin gene. [1] This was constructed from pXF3 (pBR322 minus the poison sequence), a SV40 ori, and a rat preproinsulin genomic sequence under the control of RSV LTR (pBC81delta4 cut at BglII and BalI and inserted at the BamHI and ClaI sites). [2] Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRI--2.8,0.78,0.44; HindIII--4.1; BstEII--4.1. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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