pBCL8.1


Vector IG Sequence Link :
General : plasmid ds-DNA 8100 BP
Functions : (cloning)(integrating)
Selection : ()
Copy Number :
Hosts : (Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Chromosome acentric fragmentation vector containing LYS2 for use with artificial chromosomes containing Alu repeats. pBCL8.1 should be linearized with SalI before transformation into human YAC-containing yeast strains. Lys+ Trp+ Ura- colonies should be selected. Recombinant deletion YACs can be recovered by digestion with BglII, EcoRI, HindIII, PstI, SacI or XbaI, recircularization, and transformation into E. coli. The order of the major features in the plasmid is: LYS2 - HindIII - telomere - SalI - Alu repeats - pMB1 ori - ampR - SacI - XbaI - PstI - EcoRI. [1] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: C.T.Denny
Parents : ()
Siblings : ()
Descendents : ()


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