Vector IG Sequence Link :
General : plasmid ds-DNA 4256 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : (ATCC)
Misc.Comments : Promoter-cloning vector for analysis of promoters in vertebrate cells, using the CAT reporter gene. Constructed from pBLCAT3 (ATCC37528) by deletion of a 467 bp fragment immediately upstream from the 5' polylinker and replacement by two fragments, each containing the polyadenylation signal of the SV40 large T gene. The vector contains the following restriction sites (approximate kb from nt 1): BglI--3.07; ClaI--1.69; EcoRI--0.30, 1.71; HindIII--0.0; NcoI--0.60; PvuI--3.33; PvuII--0.20, 1.89; SacI--1.70; XhoI--0.05. The order of the major features in the plasmid is: two SV40 polyadenylation signals - HindIII/MCS/XhoI - cat - SV40 small T splice site - SV40 polyadenylation signal - ClaI/MCS/SstI - pMB1 ori - ampR. [1] Deposited by: M.D.Boshart Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
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