pBM2240


Vector IG Sequence Link :
General : plasmid ds-DNA 7250 BP
Functions : (cloning)(shuttle)
Selection : ()
Copy Number :
Hosts : (Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : YC-type shuttle vector designed to clone yeast genes that are difficult to clone by rescuing inserts from the vector lambdaMG3 by homologous recombination. S. cerevisiae is cotransformed with the lambda clone of interest and vector linearized with an EcoRI/XhoI double digest. Ura+ colonies are tested for complementation of a mutant phenotype. Constructed by insertion of a 0.858 kb BamHI-XbaI fragment and a 1.5 kb KpnI fragment from lambdaMG3 into the corresponding sites of pRS316 (ATCC 77145). The order of the major features in this plasmid is: f1 ori - lacZ - T7 promoter - KpnI/lambdaMG3/KpnI - MCS - BamHI/lambdaMG3/XbaI - T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3. [1] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: M.N.Johnston
Parents : ()
Siblings : ()
Descendents : ()


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