pBM2906


Vector IG Sequence Link :
General : plasmid ds-DNA 7360 BP
Functions : (cloning)(shuttle)
Selection : ()
Copy Number :
Hosts : (Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : S. cerevisiae is cotransformed with the lambda clone of interest and vector linearized with an EcoRI/XhoI double digest. Ura+ colonies are tested for complementation of a mutant phenotype. The order of the major features in the plasmid is: f1 ori - lacZ - T7 promoter - KpnI/lambdaMG14/KpnI - MCS - BamHI/lambdaMG14/XbaI - T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3. [1] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: M.N.Johnston
Parents : ()
Siblings : ()
Descendents : ()


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