Vector IG Sequence Link :
General : plasmid ds-DNA 6200 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Trypanosoma brucei)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): HindIII, BamHI--3.5,2.7; EcoRI, BglII--5.6,0.6; BamHI--6.2; MluI--6.2. (ATCC staff) Constructed from the PARP promoter and 3' splice site from BNsp-CAT, neo (BglII/BamHI fragment) from pSV2neo, and a tubulin intergenic region (639 bp, blunted EcoRI/BglII) inserted into the SmaI site 3' to the termination codon of neo. Linearizing with MluI before transfection is essential for efficient integration. The MluI site is in the center of the tubulin intergenic sequence. Shuttle vector for integration by homologous recombination targeted to the beta- alpha-tubulin intergenic region of Trypanosoma brucei. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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