pBP108


Vector IG Sequence Link :
General : plasmid ds-DNA 4900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(Saccharomyces cerevisiae YPH252)(Saccharomyces cerevisiae CGY2516 [ATCC 74013])
Suppliers : (ATCC)
Misc.Comments : Constructed from pBP103 (ATCC 77087) by inserting a 0.3 kb Alu-containing sequence from BLUR8 into the BamHI site. pBP108 (ATCC 77088) and pBP109 (ATCC 77089) differ in the orientation of the Alu sequence. [1] Acentric chromosome fragmentation vector targeting Alu sequences. Contains promoters for in vitro RNA synthesis. [1] Any unique restriction site between the targeting sequence and the telomere-adjacent sequence can be used to linearize the plasmid before transformation. [1] The EcoRV and ClaI sites between the SP6 promoter and the telomere sequence can be used for recovery from deletion derivatives of YAC insert sequences adjacent to the fragmentation site. [1] Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 0.3; EcoRI--4.9; PstI--3.4, 1.15, 0.35. (ATCC staff) The order of the major features in this plasmid is: bla - HIS3 - lacZ'/MCS/3'Alu5' - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI - EcoRV - SP6 promoter. [1] Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
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