pBP111


Vector IG Sequence Link :
General : plasmid ds-DNA 8600 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(Saccharomyces cerevisiae YPH252)(Saccharomyces cerevisiae CGY2516 [ATCC 74013])
Suppliers : (ATCC)
Misc.Comments : Acentric chromosome fragmentation vector targeting LINE sequences. Contains promoters for in vitro RNA synthesis. [1] Constructed from pBP103 (ATCC 77087) by inserting a 4.0 kb LINE-containing SacI fragment from pL1.1A into the SacI site. pBP110 (ATCC 77090) and pBP111 (ATCC 77091) differ in the orientation of the LINE sequence. [1] Any unique restriction site between the targeting sequence and the telomere-adjacent sequence can be used to linearize the plasmid before transformation. [1] The EcoRV and ClaI sites between the SP6 promoter and the telomere sequence can be used for recovery from deletion derivatives of YAC insert sequences adjacent to the fragmentation site. [1] The order of the major features in this plasmid is: bla - HIS3 - lacZ'/MCS/3'LINE5' - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI - EcoRV - SP6 promoter. [1] Restriction digests of the clone give the following sizes (kb): ClaI--4.3 (doublet); EcoRI--7.0, 1.6; SacI--4.6, 4.0; BamHI--4.7, 2.8, 1.2. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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