Vector IG Sequence Link :
General : plasmid ds-DNA 6400 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)(Saccharomyces cerevisiae YPH252)(Saccharomyces cerevisiae CGY2516 [ATCC 74013])
Suppliers : (ATCC)
Misc.Comments : Constructed by inserting a 1.2 kb 5'-EcoRI/BamHI-3' fragment containing S. cerevisiae HIS3 into the EcoRI and BamHI sites of pSV2-neo. [1] A 0.3 kb BamHI fragment from BLUR13 was modified by adding an EcoRI site at the SphI site & then cloned into the BamHI site between neoR and HIS3. [1] If linearized with EcoRI and transformed into YAC-containing his3 deletion strains, the plasmid will target integration to Alu sequences and confer a His+ phenotype. The YAC can then be transferred to a eukaryotic cell by spheroplast fusion. [1] YI-type shuttle integrative vector to incorporate a neoR casette into human-derived (Alu-containing) YACs. [1] Restriction digests of the clone give the following sizes (kb): BamHI--6.1, 0.3; EcoRI--6.4; HindIII--3.4, 3.1. (ATCC staff) The order of the major features in this plasmid is: pMB1 ori - bla - HIS3 - BamHI - BLUR13.RI - BamHI - neoR. [1] Medium is 1227 LB plus ampicillin.
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Siblings : ()
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