pBP81


Vector IG Sequence Link :
General : plasmid ds-DNA 6200 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(Saccharomyces cerevisiae YPH252)(Saccharomyces cerevisiae CGY2516 [ATCC 74013])
Suppliers : (ATCC)
Misc.Comments : Constructed by replacing the telomere-adjacent sequence from pBP63A with a 0.25 kb SalI/SphI fragment containing the YTCA-1x telomere and CEN6 sequences. [1] Linearize with SalI (site adjacent to the telomere sequence) before transformation. [1] Contains 2 head to tail Alu sequences with the 5' end closer to the SalI site. [1] Centric chromosome fragmentation vector targeting Alu sequences. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 0.35, 0.25; PstI--4.4, 1.4, 0.4; SstI--5.4, 0.8; SalI/SphI--6.2. (ATCC staff) The order of the major features in this plasmid is: bla - HIS3 - 3'Alu5' - 3'Alu5' - SalI - TEL - CEN6. [1] Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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