Vector IG Sequence Link :
General : plasmid ds-DNA 9000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(S.cerevisiae YPH252 [ATCC 76621])(S.cerevisiae CYG2516 [ATCC 74013])
Suppliers : (ATCC)
Misc.Comments : YI-type shuttle integrative vector targeting human LINE sequences in YAC libraries derived from somatic cell hybrids. [2] Constructed by replacing a 2.2 kb XbaI fragment of the LINE element in pL1.1a with a blunt-ended 1.2 kb fragment containing HIS3. [2] Linearizing with NotI and ClaI removes plasmid sequences from the LINE ends for targeting integration. [1] The NotI site is at the 5' end of the LINE sequence. [1] The replaced element, nt 3392 to 5607 of the LINE consensus sequence, contained conserved sequences which might have permitted targeting to murine-derived YACs. [2] Restriction digests of the clone give the following sizes (kb): ClaI--9.3; HindIII--4.9, 2.55, 1.85; XbaI--uncut; NotI--9.3. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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