pBR322


Vector IG Sequence Link :
General : plasmid ds-DNA 4361 BP
Functions : (cloning general)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli C600)(E.coli RR1)(E.coli)
Suppliers : (Boehringer)(BRL)(CLONTECH)(NEB)(Pharmacia)(Promega)(Sigma)(USB)(Worthington)(ATCC)
Misc.Comments : Size is 4363 for Boehringer, BRL, Promega, Sigma. Size is 4362 for USB. Promoter P1 is artificially created by the ligation of two different DNA fragments to create pBR322. Promoter P2 in the same region as P1, but it is on the opposite strand and initiates transcription in the direction of the tetracycline resistance gene. Promoter P3 is the natural promoter for the beta-lactamase gene. Medium is 1227 LB plus ampicillin. The circular sequence is numbered such that 0 is the middle of the unique EcoRI site and the count increases first through the tet genes, the pMB1 material, and finally through the Tn3 region. NCBI gi: 58257
Parents : (pSC101)
Siblings : ()
Descendents : (M13mp9)(pAA3.7X)(pACYC177)(pAT153)(pBR325)(pBR327)(pBR328)(pCKSP6)(pEX1)(pEX2)(pEX3)(pGEM-1)(pGEM-2)(pGEM-3)(pGEM-4)(pHC79)(pi-AN7)(pi-VX)(pJRD158)(pKK223-3)(pKM1)(pKM2)(pKO-1)(pKO-2)(pKTH605)(pKTH606)(pLBU3)(pMBL1)(pMBL604)(pMC1511)(pMC1871)(pRSVneo)(pSP18)(pSP19)(pSP64)(pSP65)(pSP6-T3)(pSP6-T7-19)(pSV2cat)(pT712)(pT713)(pT7T3-18)(pT7T3-19)(pT7T3alpha-18)(pT7T3alpha-19)(pTrS3)(pUC7)(pUR222)(pUR278)(pUR288)(pUR289)(pUR290)(pUR291)(pUR292)(pV34)(YEp24)(YIp5)(YRp17)(pREV-T)(pHS102)(pEMP)(pTCT)(pSVC)(ppi12.20)(ppi25.1)(pS12.20)(pS25.1)(pPH54)(pPH507)

NCBI ENTREZ Link :


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