Vector IG Sequence Link :
General : plasmid ds-DNA 10500 BP
Functions : (cloning)(ILV2 acetohydroxyacid synthase)
Selection : ()
Copy Number :
Hosts : (Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The ClaI and HindIII sites flanking the 3.8 kb ILV2 segment were modified to XhoI sites. YI-type integrating vector containing lox sites. Inserts cloned into the BamHI or SalI sites can be excised when the Cre protein is provided in trans (see also ATCC 20772, 53255). The lox sites can be isolated by an EcoRI/SalI or an XhoI/BamHI digest. The order of the major features in this plasmid is: pMB1 ori - ampR - EcoRI - lox -> - LEU2 - lox -> - XhoI - ILV2 - XhoI. [2] Note: This material is cited in a U.S and/or other Patent and may not be used to infringe the patent claims. Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Du Pont Merck Pharmaceutical Company
Parents : ()
Siblings : ()
Descendents : ()

Return to Vector Homepage