pC4ro3


Vector IG Sequence Link :
General : plasmid ds-DNA 10800 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Drosophila melanogaster)(E.coli)(E.coli TG1)(E.coli RR1)
Suppliers : (ATCC)
Misc.Comments : Vector containing a non-autonomous P-element and incorporating the rough gene (ro) as a dominant marker for P-element mediated gene transfer into the germ line of Drosophila melanogaster. The order of the major features in this plasmid is: HindIII - P-element/Carnegie 4/P-element - XhoI/MCS/SphI - ro gene. The SphI/HindIII fragment corresponding to the rough gene contains the following restriction sites (kb from the 5' end): BamHI--3.02; BglII--5.39; EcoRI--0.91, 4.91; SacI--0.57, 4.19, 5.86; SalI--1.80, 3.91; SmaI--4.43. (personal communication) Restriction digests of the clone give the following sizes (kb): SalI--8.6, 2.2; HindIII--7.7, 3.1; PstI--10.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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