Vector IG Sequence Link :
General : plasmid ds-DNA 4986 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The component of a multiplex gene regulatory system containing the target transponding promoter sequence & reporter gene. The transactivating gene product is encoded in pNFT (ATCC 68668). Constructed by inserting the ClaI/SalI (end filled) fragment of pPO2 into the HincII site of the vector. Transcription from the SP6 promoter gives sense RNA. (personal communication) The 2.1 kb SmaI/SalI fragment contains the promoter fragment linked to coding sequences for chloramphenicol acetyltransferase (CAT) along with SV40 splice and polyadenylation signals. Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 1.6, 0.4, 0.3; PvuI/PvuII--1.8, 1.7, 1.5; SmaI/SalI--5.0; SalI--uncut; SmaI--5.0. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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