Vector IG Sequence Link :
General : plasmid ds-DNA 4650 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli AM7/pMW1)(E.coli)
Suppliers : (ATCC)
Misc.Comments : This vector contains the copA region from NR1, the copB region from Beu1, & the repA and ori sequences of NR1. There is a G/C to A/T mutation at bp 598 in the copB coding region. [2] Temperature sensitive. Grow at 28C. Ampicillin is the selection marker for pCFM526, kanamycin for pMW1. This is an expression plasmid containing a lambdaPL promoter, a ribosome binding site, a multiple cloning sequence, and a lambda Toop terminator. [2] Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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