pCGN978


Vector IG Sequence Link :
General : plasmid ds-DNA 3540 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (Agrobacterium tumefaciens K12)(Agrobacterium tumefaciens)(E.coli)(higher plants)
Suppliers : (ATCC)
Misc.Comments : Binary plasmid containing aroA in both sense (octopine synthetase or ocs promoter) and anti-sense (mas promoter) orientations. The presence of the anti-sense construct is effective in reducing aroA expression relative to controls containing the gene only in the sense orientation. USDA permit (APHIS form 2000) required for distribution. (ATCC staff) Restriction digests of the clone give the following sizes (kb): SalI--21.0, 8.8, 6.0; BglII--20.2; 15.0; EcoRI--22.0, 4.4, 4.0, 3.4, 1.6; BamHI--25.6, 8.0, 1.6; ClaI--22.0, 6.5, 2.6, 2.4, 1.8. (ATCC staff) Medium is -1 1065 plus kanamycin (50 ug/ml) plus streptomcyin (20 ug/ml).
Parents : ()
Siblings : ()
Descendents : ()


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