pCHR81


Vector IG Sequence Link :
General : plasmid ds-DNA 4000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli MC1061)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Replication is temperature-sensitive. Constructed by mutagenizing R388 to construct a temperature sensitive derivative and then transposing on Tn5. Can be used as a suicide vector for the delivery of transposable elements. Restriction digests of the clone give the following sizes (kb): BamHI--32, 4.0, 1.8; PstI--36, 2.7, 1.8; HindIII--38, 3.8, 3.0. (ATCC staff) Medium is 1236 LB plus kanamycin.
Parents : ()
Siblings : ()
Descendents : ()


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