pCLL34


Vector IG Sequence Link :
General : plasmid ds-DNA 19800 BP
Functions : (cloning)(promoter-cloning)
Selection : ()
Copy Number :
Hosts : (Streptomyces)
Suppliers : (ATCC)(American Cyanamid)
Misc.Comments : Promoter-cloning vector permitting visual detection of activity by expression of a brown pigment. pCLL34 is a promoter-cloning vector derived from pARC1 by the insertion into the BamHI site of a 844 bp BclI fragment from pIJ486 containing the fd terminator and a polylinker. Constructed by inserting a 844 bp BclI fragment from pIJ486 containing the fd terminator and a polylinker into the BamHI site of pARC1. The additional 35 bp of MCS between the BamHI site and the start of the brown genes do not interfere with gene expression. Restriction digests of the clone give the following sizes (kb): EcoRI--16.71, 3.41. The order of the major features in this plasmid is: fd terminator - MCS - brown genes - tsrR. (U.S. Patent No. 4,994,389 dated Feb. 19, 1991) Note: This material is cited in a U.S and/or other Patent and may not be used to infringe the patent claims. Deposited by: American Cyanamid Growth media: R2 YE medium (ATCC medium number 1339) MCS. not original sequence.
Parents : (pARC1)(pIJ486)
Siblings : ()
Descendents : ()


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