Vector IG Sequence Link :
General : plasmid ds-DNA 9910 BP
Functions : (cloning)(shuttle)
Selection : (mel tyrosinase)
Copy Number :
Hosts : (E.coli)(E.coli W5445)(Actinomadura kijaniata)(Streptomyces lividans)
Suppliers : (ATCC)(American Cyanamid Company)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI--10.2; BglII--10.2; PstI--10.2. (ATCC staff) Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli. Cloning into the BglII, SphI, or SstI sites inactivates the tyrosinase gene. The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora. [1] Note: This material is cited in a U.S and/or other Patent and may not be used to infringe the patent claims. Growth: LB plus ampicillin (ATCC medium number 1227) 37C
Parents : ()
Siblings : ()
Descendents : ()

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