Vector IG Sequence Link :
General : plasmid ds-DNA 9000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. Presence of alcohol dehydrogenase activity is used to follow cell lineage in culture or in situ. The order of the major features in this plasmid is:CMV promoter - Drosophila ADH-s - SV40 polyadenylation signal - RSV promoter - neoR - polyoma ori - SV40 ori - pMB1 ori. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; HindIII/XbaI--7.2, 1.8; SmaI--9.2; StuI--9.2. (ATCC staff) Medium is 1799 LB plus neomycin (10 ug/ml).
Parents : ()
Siblings : ()
Descendents : ()

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