Vector IG Sequence Link :
General : plasmid ds-DNA 7164 BP
Functions : (expression)(reporter)(promoter analysis)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(monkey COS cells)(vertebrate cells)(E.coli)
Suppliers : (CLONTECH)(ATCC)
Misc.Comments : ATCC size is 7300 bp. The order of the major features in this plasmid is [1]: pUC19 - EcoRI - CMV immediate early promoter/enhancer - SV40 late 16S/19S splice signals -NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Designed to express high levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. [1] Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; HindIII--7.3; EcoRI/HindIII--4.6, 2.7; NotI--3.8, 3.5; BamHI--3.65; 3.45; 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml).
Parents : ()
Siblings : (pADbeta)(pNASSbeta)(pSVbeta)(pTKbeta)
Descendents : ()

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