pCMVlacZII


Vector IG Sequence Link :
General : plasmid ds-DNA 10900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ. The order of the major features in this plasmid is:CMV promoter - bacterial lacZ - polyadenylation signal - RSV promoter - neoR - polyoma virus ori - SV40 ori - pMB1 ori - M13 ori. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; HindIII/XbaI--7.2, 3.8; KpnI--7.4, 3.6; StuI--11.0. (ATCC staff) Medium is 1799 LB plus neomycin (10 ug/ml).
Parents : ()
Siblings : ()
Descendents : ()


Return to Vector Homepage