Vector IG Sequence Link :
General : plasmid ds-DNA 4100 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (Pseudomonas aeruginosa PAO1024 [ATCC 47053])(Pseudomonas)(P.aeruginosa PAO1162)(P.putida KT2440 [ATCC 47054])
Suppliers : (ATCC)
Misc.Comments : Constructed by inserting a 0.9 kb PstI fragment from R1 into the PstI site of pUC7, cloning a 0.9 kb BamHI fragment from this intermediate into the BamHI site of pCN38 (ATCC 77095), and deleting a 0.9 kb PstI fragment. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536 between the repA promoter and coding sequences. (personal communication) Generates the equivalent of pCN38 with part of the multiple cloning site of pUC7. General purpose vector. (ATCC staff) Restriction digests of the clone give the following sizes (kb): BamHI--4.4; KpnI/PvuII--2.6, 1.6; HindIII/PstI--2.8, 1.4. (ATCC staff) The order of the major features in this plasmid is:PvuII - MCS - kanR - HindIII - KpnI - repA. Medium is 1236 LB plus kanamycin.
Parents : ()
Siblings : ()
Descendents : ()

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