pCN41


Vector IG Sequence Link :
General : plasmid ds-DNA 4500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (Pseudomonas aeruginosa PAO1024 [ATCC 47053])(Pseudomonas)(P.aeruginosa PAO1162)(P.putida KT2440 [ATCC 47054])
Suppliers : (ATCC)
Misc.Comments : Constructed by excising a BamHI/HindIII fragment encoding part of kanR from pCN40 (ATCC 77097), blunting with Klenow fragment, and religating. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536 between the repA promoter and coding sequences. (personal communication) General purpose vector. (ATCC staff) Restriction digests of the clone give the following sizes (kb): EcoRI--2.4, 1.1, 1.0; KpnI/SmaI--4.6; PvuII/PstI--2.5, 2.0; KpnI--4.6; SmaI--uncut. (ATCC staff) Medium is -1 1065 plus streptomycin.
Parents : ()
Siblings : ()
Descendents : ()


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