pCN50


Vector IG Sequence Link :
General : plasmid ds-DNA 8500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Pseudomonas aeruginosa)(Pseudomonas putida)(E.coli)(P.aeruginosa PAO1162)(P.aeruginosa PAO1024)(P.putida KT2440 [ATCC 47054])
Suppliers : (ATCC)
Misc.Comments : Multiple selectible markers permits insertional activation to screen for recombinants (PstI site in ampR). Constructed by ligating pCN38 (ATCC 77095) and pBR322 which had been linearized with PvuII. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536 between the repA promoter and coding sequences. (personal communication) Retains the mobilization site of pBR322-derived vectors and can be mobilized if suitable functions are provided in trans. Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 3.1; HindIII/PstI--4.5, 3.4, 0.8; PvuII--4.4 (doublet); PvuII/KpnI--4.4, 2.5, 1.6. (ATCC staff) The order of the major features in this plasmid is:PvuII - pBR322 (pMB1 ori - bla - tetR) - PvuII - BamHI - kanR - HindIII - repA. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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