pCN60


Vector IG Sequence Link :
General : plasmid ds-DNA 8700 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Pseudomonas aeruginosa)(Pseudomonas putida)(E.coli)(Pseudomonas aeruginosa PAO1024)(Pseudomonas aeruginosa PAO1162)(P.putida KT2440)
Suppliers : (ATCC)
Misc.Comments : Constructed by ligating pCN40 (ATCC 77097) and pUC18, each linearized with KpnI. Shuttle vector. (ATCC staff) Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536 between the repA promoter and coding sequences. (personal communication) Retains the mobilization site of pBR322-derived vectors and can be mobilized if suitable functions are provided in trans. Restriction digests of the clone give the following sizes (kb): HindIII--8.2, 0.9; KpnI--6.0, 2.8; PstI--6.6, 2.4; XbaI--9.4; SmaI--7.6, 1.1. (ATCC staff) The order of the major features in this plasmid is:KpnI - repA - strR - PstI - SmaI - BamHI - kanR - HindIII - KpnI - MCS - HindIII - bla - pMB1 ori. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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