pCPOT


Vector IG Sequence Link :
General : plasmid ds-DNA 11700 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): PstI--14, 4.3; AccI--3.3, 2.7, 2.7, 2.5, 2.5, 2.2, 1.5, 1.3; BglI--11.0, 4.5, 2.5; EcoRI--4.2, 3.3, 2.9, 2.9, 1.7, 1.3, 1.3. (ATCC staff) The presence of the POT1 gene ensures stable maintenance of the plasmid in yeast during growth on nonselective medium using glucose as a carbon source. [1] An episomal Saccharomyces cerevisiae/E.coli shuttle expression vector containing 2 micron circle, pBR322 replication and selection, and Schizosaccharomyces pombe sequences encoding triose phosphate isomerase (POT1). [1] Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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