pCS19


Vector IG Sequence Link :
General : plasmid ds-DNA 9344 BP
Functions : (cloning)(shuttle)(positive selection for inserts)
Selection : ()
Copy Number :
Hosts : (E.coli)(Saccharomyces)
Suppliers : (ATCC)
Misc.Comments : Shuttle vector for construction of genomic libraries that permits direct selection of recombinants. Insertion into the BclI site inactivates the cI repressor, allowing expression of tetracycline resistance in recombinants. Propagation in a dam- host is necessary for cleavage at the BclI site. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--5.2, 6.4; BglII--0.6; EcoRI--0.0, 4.46; HindIII--0.84, 2.0, 4.21, 4.33; PstI--0.63, 1.8, 8.59; SalI--5.48; SmaI--0.9, 4.16; XbaI--2.43; XhoI--3.75. The order of the major features in the plasmid is: EcoRI - ARS1 - SmaI - URA3 - XbaI - CEN15 - XhoI - BclI/cI - lambda PR - tetR - trp terminator - BamHI - pMB1 ori - ampR. [2] Growth medium: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: C.G.Sengstag
Parents : ()
Siblings : ()
Descendents : ()

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