Vector IG Sequence Link :
General : plasmid ds-DNA 6360 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse cell line C11D)
Suppliers : (ATCC)
Misc.Comments : The BamHI and EcoRI sites are 3' of the unique available in-phase initiation codon under control of the SV40 early promoter. Constructed by inserting the PvuII/BamHI fragment from pSV2-cat (SV40 promoter, small-t intron, polyadenylation signal,inactive CAT) into pUT322 (2 micron origin, URA3). Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BamHI/PvuII--5.6, 0.5; EcoRI--6.0. (ATCC staff) Shuttle expression vector. (ATCC staff) Permits expression in both S. cerevisiae and mouse cells. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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