pCYM00


Vector IG Sequence Link :
General : plasmid ds-DNA 9408 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
Suppliers : (ATCC)
Misc.Comments : Constructed by inserting a BamHI/SalI fragment from pMC1403 (lac operon beginning at codon 9 of lacZ) between the BamHI/BalI (SalI-linkered) sites of pCYM0. Expresses beta-galactosidase activity in E.coli, S. cerevisiae, and mouse cells, and thus can be used as a control to calibrate transformation efficiency. Restriction digests of the clone give the following sizes (kb): BamHI/SalI--6.0(doublet); EcoRI--12.0; SmaI--12.0; Pvu/BamHI--6.5, 2.5, 2.1, 0.54, 0.49. (ATCC staff) Our plasmid is 12 kb instead of 9.4, which matches the original depositor's DNA. (ATCC staff) Shuttle reporter plasmid. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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