pCYM01


Vector IG Sequence Link :
General : plasmid ds-DNA 8745 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
Suppliers : (ATCC)
Misc.Comments : The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Expresses beta-galactosidase activity in E.coli, S. cerevisiae, and mouse cells, and thus can be used as a control to calibrate transformation efficency. Constructed by inserting a 3.048 kb EcoRI/DraI fragment from pMC1403 into pCYM1 cut with BamHI (nt 427), linkered to add an EcoRI site, and cut with BalI (nt 702). Restriction digests of the clone give the following sizes (kb): BamHI/PvuII--4.8, 2.6, 0.5, 0.46; EcoRI--8.4; SmaI--8.4. (ATCC staff) Also permits blue/white detection in E.coli. Shuttle expression vector which includes a reporter group. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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