pCYM1-11


Vector IG Sequence Link :
General : plasmid ds-DNA 5639 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
Suppliers : (ATCC)
Misc.Comments : The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Constructed by replacing the HindIII/EcoRI fragment of pCYM1 with the HindIII/EcoRI multiple cloning site from M13mp11. The translation start codon has been removed by this process. Restriction digests of the clone give the following sizes (kb): BamHI--5.6; BamHI/PvuII--5.2, 0.5; EcoRI--5.6. (ATCC staff) Shuttle expression vector. (ATCC staff) Permits expression in both S. cerevisiae and mouse cells. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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