pCYM2


Vector IG Sequence Link :
General : plasmid ds-DNA 5462 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
Suppliers : (ATCC)
Misc.Comments : The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Constructed by deleting the HpaI (nt 1686)/BglII (nt 3463) site from pCYM0 and inserting the 879 bp BglII/EcoRI fragment containing TRP1 from YRp7. Restriction digests of the clone give the following sizes (kb): BamHI--5.2; BglII/EcoRI--3.2, 2.0; EcoRI--5.2; BamHI/PvuII--4.5, 0.51. (ATCC staff) Shuttle expression vector. (ATCC staff) Permits expression in both S. cerevisiae and mouse cells. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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