pCYM3


Vector IG Sequence Link :
General : plasmid ds-DNA 6543 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
Suppliers : (ATCC)
Misc.Comments : The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Shuttle expression vector. (ATCC staff) Permits expression in both S. cerevisiae and mouse cells. (ATCC staff) Constructed by inserting a 1.207 kb ClaI/BamHI fragment containing URA3d into pCYM2 cut with BglII & ClaI (nt 3469 and 3589 according to the pCYM0 numbering scheme). URA3d is a modified URA3 gene which is not efficiently expressed and requires a large number of plasmids to complement a ura3 mutation. Restriction digests of the clone give the following sizes (kb): BglII--uncut; ClaI/BamHI--3.4,2.8,0.5; EcoRI--6.6; BamHI/PvuII--6.0, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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