Vector IG Sequence Link :
General : plasmid ds-DNA 110 BP
Functions : (cloning)(expression eukaryote)
Selection : ()
Copy Number :
Hosts : (E.coli K-12)(E.coli RV308)
Suppliers : ()
Misc.Comments : lpp promoter DNA from pIMIA. bovine cDNA provided by J.D.Baxter, grown in E.coli K-12 (RV308 cells), clone pCZ101. [1] constructed expression plasmids to get more efficient production of growth hormone (bGH). Besides an efficient promoter (lpp) additional codons in the 5' end of the growth hormone coding sequence were required to raise the production level. An artificial sequence (bp 23-109) was inserted between the XbaI and HgiAI sites, of which the first eight codons after the bGH start codon are foreign to the natural gene. When the number of artificial codons directly 3' to the 'atg' codon was decreased, the amount of growth hormone produced also decreased dramatically. Bases 42-65 are missing in pCZ108 and pCZ104 and are replaced by 'gct' in pCZ100. Variations, as annotated in the Sites Table also caused different levels of bGH production. NCBI gi: 208979
Parents : (pIMIA)(bovine bGH cDNA)
Siblings : (pCZ108)(pCZ104)(pCZ100)
Descendents : ()


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