Vector IG Sequence Link :
General : plasmid ds-DNA 98 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : ()
Misc.Comments : E.coli trp promoter DNA from pHI7. bovine cDNA provided by J.D.Baxter, grown in E.coli K-12 (RV308 cells), clone pCZ105. [1] constructed expression plasmids to get more efficient production of growth hormone (bGH). Besides an efficient promoter (trp) additional codons in the 5' end of the growth hormone coding sequence were required to raise the production level. An artificial sequence (bp 22-97) was inserted, between the TaqI and HgiAI sites of which the first eight codons after the bGH start codon were foreign to the natural gene. When the number of artificial codons just 3' of the 'atg' codon was decreased, the amount of growth hormone produced also decreased dramatically. Bases 30-53 are missing in pCZ110, and replaced by 'gat' and 'gatgataag' in pCZ115 and pCZ112, respectively. Variations, as annotated in the Sites Table, also caused different levels of bGH production. NCBI gi: 208981
Parents : (pHI7)
Siblings : (pCZ110)(pCZ115)(pCZ112)
Descendents : ()


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