pDA71


Vector IG Sequence Link :
General : plasmid ds-DNA 8800 BP
Functions : (cloning)(shuttle)(library)(hsdR restriction endonuclease EcoRI)
Selection : ()
Copy Number :
Hosts : (Rhodococcus)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Shuttle vector permitting positive selection in E. coli. Cloning sites are positioned in the EcoRI endonuclease gene, regulated by a lambda promoter. In E. coli hosts providing the temperature-sensitive lambda repressor cI857, growth at 37C will occur only if the endonuclease gene is interrupted. The non-enzymatic chloramphenicol resistance gene, derived from Rhodococcus rhodochrous SC2318 (ATCC 12674), does not confer resistance in E. coli. The plasmid confers immunity to nocardiophage Q4. [1] Growth media: LB plus ampicillin (ATCC medium number 1227) 30C Deposited by: E.R.Dabbs
Parents : ()
Siblings : ()
Descendents : ()


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