Vector IG Sequence Link :
General : plasmid ds-DNA 6100 BP
Functions : (cloning)(shuttle - integrating)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli DH5alpha)(Saccharomyces cerevisiae)(vertebrate cells)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI--5.8, 0.3; PvuI--6.1; EcoRI--4.0, 2.3. (ATCC staff) YI-type shuttle integrative vector useful for incorporating a neoR cassette into YACs. Constructed by ligating the 1.35 kb XhoI fragment of pPOL2shortneobpA to the largest PvuII fragment of pBP47. Linearizing with SmaI and transformating into a YAC-containing his3 deletion strain will target integration to Alu sequences. Linearizating at the PvuI site will target integration to the TRP1 centric arm of pYAC4 derivatives. His+ transformants can then be transferred to a eukaryotic cell by spheroplast fusion. The order of the major features in this plasmid is: pMB1 ori - bla - HIS3 - BamHI - SmaI - BamHI - pol2sneo. (personal communication) Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Reeves R.H.
Parents : ()
Siblings : ()
Descendents : ()

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