pDdGal-15


Vector IG Sequence Link :
General : plasmid ds-DNA 8500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Dictyostelium discoideum)(E.coli SURE)(E.coli DH5)
Suppliers : (ATCC)
Misc.Comments : pDdGal-15 (ATCC 37770), pDdGal-16 (ATCC 37771) and pDdGal-17 (ATCC 37772) differ in their polylinker sequences. Each contains a BglII site in a different reading frame relative to the lacZ gene. A promoter-cloning plasmid vector using expression of beta-galactosidase as the reporter in Dictyostelium. The lacZ gene and Dictyostelium actin 8 terminator from pDdlac-1 were inserted into a derivative of pB10TP2 from which the f1 origin and and a lacZ fragment had been removed. The neo gene is regulated by an actin 6 promoter and a CP1 terminator. Restriction digests of the clone give the following sizes (kb): EcoRI--8.5; BamHI--8.5; BglII--8.5. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pDdlac-1)(pB10TP2 from pEMBL18+)
Siblings : ()
Descendents : ()


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