p-delta-blue


Vector IG Sequence Link :
General : plasmid ds-DNA 3507 BP
Functions : (cloning)(expression)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)
Suppliers : ()
Misc.Comments : The insert to be cloned should have a phosphorylated 5' blunt end with the first base corresponding to the first base of the first codon. (personal communication) Expression vector producing proteins with the one additional N-terminal methionine. A modified lacZ' allows blue/white screening of colonies.[1] Constructed from pfXblue (ATCC 77333) by deletion of lambda cII and coagulation factor Xa protease recognition sequences (nt 824-943). [1] Inserts can be cloned into the vector using a filled-in NcoI site and a MCS restriction site matching the 3' end of the insert. The vector supplies the AUG initiator codon. (personal communication) The order of the major features of the plasmid is: pMB1 ori - ampR - lambdaPL promoter - NcoI/MCS/HindIII/modified lacZ'. [1] Restriction digests of the plasmid give the following sizes (kb): BamHI--3.507; BglI--1.784, 1.723; PstI--2.072, 1.435; PvuI--2.063, 1.444; PvuII--2.852, 0.655; SacI--3.257, 0.25. (personal communication) Restriction digests of the clone give the following sizes (kb): BglI--1.75 (doublet); EcoRI--3.5; PstI--2.1, 1.45. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 208983
Parents : (pfXblue)
Siblings : ()
Descendents : (phFR also from pcIIhFR)(phFR18+ from pUC/AR18+)

NCBI ENTREZ Link :


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