pDG1


Vector IG Sequence Link :
General : cosmid ds-DNA 12200 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Aspergillus niger)(E.coli)(Aspergillus niger 350-52 [ATCC 20739])
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--9.4,1.5,1.3; EcoRI/BamHI--6.3, 5.9; PstI--10.2, 2.0. (ATCC staff) The argB BamHI/EcoRI fragment contains the following sites (separated by approximate kb): BamHI - 0.8 - SalI - 0.4 - HindIII - 1.4 - SalI - 0.1 - HindIII - 0.7 - SalI - 0.1 - HindIII - 0.7 - SalI - 0.7 - SalI - 0.5 - EcoRI. [2] This was constructed by inserting the 5.0 kb EcoRI/HindIII fragment from pBB116 containing argB into pHC79 cut with EcoRI + HindIII. [1] This is an integrative, positive selection vector conferring arginine prototrophy on auxotrophic host such as Aspergillus niger 350(-)52 (ATCC 20739) (argB-). [1] Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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