pDG3


Vector IG Sequence Link :
General : plasmid ds-DNA 7800 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli JM83)(Aspergillus niger)(E.coli)(Aspergillus niger 350-52 [ATCC 20739])
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): HindIII/SalI--3.1, 1.65, 1.3, 0.75, 0.5 + smaller; XbaI--4.4, 3.4; EcoRI--7.8; SmaI--4.3, 3.5; SphI--7.8. (ATCC staff) This was constructed by inserting a HindIII/SalI fragment of Tn5 into pUC12 cut with HindIII/SalI (pNE02) and then inserting an XbaI fragment from pBB116 into the XbaI site of pNE02. [1] This is an integrative, selectable vector conferring arginine prototrophy on arginine autotrophic hosts such as Aspergillus niger 350(-)52, ATCC 20739 (argB-). It also confers kanamycin resistance.[1] Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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