pDK101


Vector IG Sequence Link :
General : phagemid ds-DNA 3027 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli JM109)
Suppliers : (ATCC)
Misc.Comments : Deposited by: Bernard Weisblum Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0. (ATCC staff) Vector useful for cloning-double stranded amplification products. Permits synthesis of ssDNA and contains promoters for in vitro RNA synthesis. [1] The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014. [1] The order of the major features in this plasmid is: T7/ApaI/MCS/NsiI/lacZ'SP6 - pMB1 ori - pMB1 - ampR - f1 ori. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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