pDUAL)(pJANUS


Vector IG Sequence Link :
General : plasmid ds-DNA 7900 BP
Functions : (cloning)(RNA synthesis in vitro)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : ()
Misc.Comments : Cosmid cloning vector for generating nested deletions and sequencing templates in both directions in vivo. Based on the transposon gamma-delta (Tn1000), and contains paired selectable and counterselectable markers. Deletions of a pDUAL clone can be obtained by transforming into a host expressing the gamma-delta transposase, such as E. coli CBK884(pXRD4043) (ATCC 47073), followed by antibiotic selection and sensitivity screening. Deletion derivatives may be sequenced from the SP6 or T7 promoters engineered within the ends of the gamma-delta component. The order of the major features is: RsrII - SP6 promoter - Tn end - sacB (sucrose sensitivity) - kanR - (lacZalpha - EcoRI/MCS/HindIII - lacZalpha) - tetR - cos - rpsL/strA (strS) - Tn end - T7 promoter - NotI - pMB1 ori - Tn1000 - ampR. [1] Deposited by: C.M.Berg Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
Parents : (pXRD4043)(pACYC184)
Siblings : ()
Descendents : ()


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