pECV25


Vector IG Sequence Link :
General : plasmid ds-DNA 11317 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)(HeLa cells)
Suppliers : (ATCC)
Misc.Comments : The order of the major features in this plasmid is:bla (ampR) - hph (hygR) - RSV LTR - HindIII - rabbit beta-globin intron splicing signals - NotI - agpt (G418R) - XhoI - rabbit beta-globin polyadenylation signal - SalI - oriP - SacI - EBNA-1 - ClaI. [3] Shuttle expression vector maintained episomally in human cells at a copy number of 25 - 50. Shows high transfection efficiency and can be introduced as supercoiled plasmids. [3] The EBNA-1 gene contains an in-frame deletion of a repetitive region of 700 +/- 20 bp. This deletion increases the number of stably maintained plasmid molecules per cell. [3] Vector can be rescued from transfected cells with no detectable rearrangements. [3] Restriction digests of the clone give the following sizes (kb): HindIII/SacI--5.7 (doublet); SalI--11.5; XhoI--11.5. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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