pEG5005


Vector IG Sequence Link :
General : plasmid ds-DNA 10200 BP
Functions : (cloning)(integrating)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli KB358)
Suppliers : (ATCC)(Omnigene, Inc.)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): ClaI--10.2,; EcoRI--8.8, 1.5; HindIII--5.2, 5.0. (ATCC staff) Mini-bacteriophage Mu-based integration vector (transposon) with a pMB1 replicon. Defective for morphogenic functions. To maintain the plasmid, grow at 30C with ampicillin and kanamycin selection. Thermal induction (42C) of the Mu cts lysogen containing the plasmid yields a low titer phage lysate. Lysates should be used within 1 week of preparation since the clones can be unstable. Infection of sensitive, Mu cts lysogenic E. coli strains and growth at 30C yields two types of kanamycin-resistant isolates: ampicillin-sensitive strains (integrants) that carry no plasmid DNA, and ampicillin-resistant strains that contain the plasmid. [1] Note: This material is cited in a U.S and/or other Patent and may not be used to infringe the patent claims. Growth: LB plus amp (50ug/ml) plus kan (20ug/ml)(ATCC med num 1948)30C
Parents : ()
Siblings : ()
Descendents : ()


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