pEMBL8-


Vector IG Sequence Link :
General : phasmid ds-DNA 3939 BP
Functions : (transcription)
Selection : ()
Copy Number :
Hosts : (E.coli 71/18)(E.coli)(E.coli JM series)
Suppliers : (ATCC)
Misc.Comments : According to the cloning strategy, the phage f1 origin should be flanked by regenerated EcoRI sites, but these are not found experimentally. The given sequence must be incorrect around this position. The base n was introduced to destroy the EcoRI site (GAAnTTC). ssDNA-producing plasmid with polylinker in lacZ'. Medium is 1227 LB plus ampicillin.
Parents : (pUC8)(M13mp8)
Siblings : (pEMBL8+)
Descendents : ()

NCBI ENTREZ Link :


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