pEX12


Vector IG Sequence Link :
General : plasmid ds-DNA 5808 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli POP2136 [ATCC 47049])(E.coli)
Suppliers : (Biores)(ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. This strain is resistant to 100 ug/ml ampicillin. (personal communication) This is one of three bacterial expression vectors (pEX11, ATCC 37708; pEX12, ATCC 37709; and pEX13, ATCC 37710) which differ by translational reading frames and which express cro-lacZ fusion proteins regulated by the PR promoter of bacteriophage lambda. [2] This was constructed from pEX2 by ligating a synthetic oligonucleotide into pEX2 cut with EcoRI and BamHI. The oligonucleotide added several unique restriction sites. [1] The recombinant fusion protein generated using this vector is not solubilized in Triton X-100 lysis procedures and is thus easily purified, resistant to proteolysis, and easily detected by direct immunoscreening. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--5.8; BamHI--5.8; PstI--5.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pEX2)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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